Uploaded on Apr 27, 2023
PPT on Colorimetry
Colorimetry
COLORIMETRY INTRODUCTION Colorimetry has been around since the 1870s when Jules Dubosq invented the colorimeter. In 1885 Joseph William Lovibond devised a method of measuring colors with a handheld comparator while working in his father's brewery. A TECHNIQUE Colorimetry is a technique for measuring the concentration of a known constituent in a solution compared to standard solutions. ABOUT COLORIMETRY The human eye perceives light and color in a very subjective way. Science demands an objective way of measuring things, which is colorimetry. Mathematically, the Beer-Lambert Law, which is a combination of two laws, defines colorimetry. BEER-LAMBERT LAW Beers Law says that if monochromatic light passes through a homogenous solution, the amount of light transmitted is directly proportional to the solute in the solution. Lamberts Law states that the length of the path determines the absorbance of light by a colored solution and the volume of liquid it passes through COLORIMETRY USES BEER-LAMBERT LAW Colorimetry uses this law to measure the absorbance (more frequently) or light transmittance to determine solution concentrations. The solution must not fluoresce, and there should be no temperature change during the measuring process. COLOR COMPARATORS AND COLORIMETERS The principle of Lovibond's comparator is still in use today, and indeed the "Lovibond Comparator" is still a brand of the Tintometer company in the UK. This type of colorimeter, sometimes called a "Filter Photometer," consists of a handheld cube with space for two cuvettes into which liquids for analysis are placed. AN ILLUMINANT A specific light source (daylight in a comparator), usually an LED or incandescent light, which projects a fixed, consistent light onto an object. AN OBSERVER A specified field of view to analyze colors. A standard observer is usually two degrees in a colorimeter USES OF COLORIMETRY Colorimetry is used in monitoring to measure bacterial growth rates, quality, and color in food and beverage manufacturing, textiles manufacturing, cosmetics, paint works, and the petroleum industry. THANK YOU
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