Colorimetry


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Uploaded on Apr 27, 2023

Category Education

PPT on Colorimetry

Category Education

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Colorimetry

COLORIMETRY INTRODUCTION  Colorimetry has been around since the 1870s when Jules Dubosq invented the colorimeter.  In 1885 Joseph William Lovibond devised a method of measuring colors with a handheld comparator while working in his father's brewery. A TECHNIQUE  Colorimetry is a technique for measuring the concentration of a known constituent in a solution compared to standard solutions. ABOUT COLORIMETRY  The human eye perceives light and color in a very subjective way. Science demands an objective way of measuring things, which is colorimetry.  Mathematically, the Beer-Lambert Law, which is a combination of two laws, defines colorimetry. BEER-LAMBERT LAW  Beers Law says that if monochromatic light passes through a homogenous solution, the amount of light transmitted is directly proportional to the solute in the solution.  Lamberts Law states that the length of the path determines the absorbance of light by a colored solution and the volume of liquid it passes through COLORIMETRY USES BEER-LAMBERT LAW  Colorimetry uses this law to measure the absorbance (more frequently) or light transmittance to determine solution concentrations.  The solution must not fluoresce, and there should be no temperature change during the measuring process. COLOR COMPARATORS AND COLORIMETERS  The principle of Lovibond's comparator is still in use today, and indeed the "Lovibond Comparator" is still a brand of the Tintometer company in the UK.  This type of colorimeter, sometimes called a "Filter Photometer," consists of a handheld cube with space for two cuvettes into which liquids for analysis are placed. AN ILLUMINANT  A specific light source (daylight in a comparator), usually an LED or incandescent light, which projects a fixed, consistent light onto an object. AN OBSERVER  A specified field of view to analyze colors. A standard observer is usually two degrees in a colorimeter USES OF COLORIMETRY  Colorimetry is used in monitoring to measure bacterial growth rates, quality, and color in food and beverage manufacturing, textiles manufacturing, cosmetics, paint works, and the petroleum industry. THANK YOU